Reporter

Part:BBa_K1555000:Design

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-09-28)


The copa promoter wth a gfp reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914


Design Notes

For guaranteeing our sensor is simple but copper-sensitive,we select upstream 17bp-249bp of copa promoter as our candidate.It includes a sequence which indirectly regulated by copper through copper-responsive protein CueR.

Source

We attained the part of copa from E.coli's genome.

References

Outten, F. W., Outten, C. E., Hale, J., & O'Halloran, T. V. (2000). Transcriptional Activation of an Escherichia coli Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR. Journal of Biological Chemistry,275(40), 31024-31029.